Composite

Part:BBa_K2771055

Designed by: Fabio Nunes de Mello   Group: iGEM18_USP-Brazil   (2018-10-12)


Rpa constitutive Receiver

Quorum sensing is a bacterial communication system that can be very useful for bacterial communication in synthetic biology. In this system, AHL (N-Acyl homoserine lactone) signaling molecules generated in a bacteria binds to a receiver in other bacteria to activate downstream gene expression. AHL molecules are generated by a synthase and received by other bacteria through a reporter.

Among all the QS pathways, the Rpa pathway is based on the production of the 3-phenol-HSL (homoserine lactone) produced by the Rpal protein an received by the RpaR protein. This part contains the RpaR transcribed by a Andersen family constitutive promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference


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Categories
Parameters
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